Plasmid pBABE-puro SV40 LT from Dr. Thomas Roberts's lab contains the insert SV40 LT and is published in Cancer Cell. 2003 May . 3(5):483-95. This plasmid is available through Addgene.

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TAg is a product of an early gene transcribed during viral infection by SV40, and is involved in viral genome Domains. The TAg has a CUL7 -binding domain, a TP53 -binding domain, a Zinc finger, and a Superfamily 3 ATPase/Helicase Mechanism. After entering the The SV40 T antigen is encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition, the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific All lanes : Anti-SV40 T-antigen antibody [PAb416] (ab16879) at 1 µg/ml Lane 1 : Whole cell lysate mouse embryonic fibroblasts immortalized by transduction with a virus harboring the gene encoding the large T antigen. Lane 2 : Whole cell lysate mouse embryonic fibroblasts, not immortalized.

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The T-antigen was assayed by complement fixation, and the TSTA was assayed by its ability to immunize mice against SV40-containing ascites tumor cells. When T-antigen- and TSTA-containing preparations were sedimented through sucrose gradients, each antigen had a major peak of activity at a sedimentation coefficient of 6.7 and minor peaks in other regions.

Rep proteins are normally synthesized by AAV-infected cells only in the presence of adenovirus. SV40 Tantigen contains a helicase activity that, in the presence of a single-stranded DNA binding protein, can extensively unwindthe template DNA.Inclusion ofE.

SV40 T-antigen from simian virus (insect cells, ≥ 80% SDS-PAGE); The large T antigen (TAg) of Simian virus 40 acts as an initiator of DNA replication, participates in …

The ELISA (enzyme-linked immunosorbent assay) is a widely used application for detecting and quantifying proteins and antigens from various samples. Target-specific ELISA kits are available from a variety of manufacturers … Adeno-associated virus (AAV) inhibits the induction of host DNA synthesis by simian virus 40 (SV40) large-tumour (T) antigen, mediated through AAV-encoded 'Rep' regulatory proteins.

80. 104,00 kr. IgG1/G3. 160 SV40 DNA. 430. 559,00 kr. Puumala. Puumala RNA. 510.
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Culture medium: Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal calf serum, 100 U/ml penicillin and 100 μg/ml streptomycin. SV40 T-antigen transformed nasopharyngeal epithelial cells contained free, wild-type p53. Moreover, LMP1 regulated p53 both at transcriptional and translational level.

Validated in IHC and tested in Simian Virus 40. This Anti-SV40 T Antigen (Ab-2) Mouse mAb (PAb416) is validated for use in Affinity Purification, Immunoblotting, IF, IP, Paraffin Sections for the detection of Abstract.
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Large T antigen. Gene. N/A. Organism. Simian virus 40 (SV40) Status. Unreviewed-Annotation score: -Protein predicted

124,80 kr. DAT per testreagens. 80. 104,00 kr.

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Gömmer sina antigen. * Under ett sockerlager (kapsel) många bakterier ex Streptocker och Staffylokocker och många andra. * Genom att gå in i våra celler.

TAg is capable of inducing malignant transformation of a variety of cell types. Simian virus 40 (SV40) is a small, DNA-containing tumour virus. One of its gene products, the large tumour antigen (T-ag), is essential for both viral replication and cell transformation. SV40 T-ag can be considered a dual oncogene protein; it is a composite transforming protein that provides distinct functions at different subcellular locations.